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All Journal Jurnal Pendidikan dan Pembelajaran Prosiding Seminar Biologi Indonesian Journal of Biotechnology Jurnal Teknologi Laboratorium Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Gorontalo Journal of Public Health
Sri Darmawati
Muhammadiyah Semarang University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Journal : Jurnal Teknologi Laboratorium

 1 
IDENTIFIKASI KONTAMINASI Salmonella Typhi BERDASARKAN AMPLIFIKASI GEN fliC PADA CINCAU DI PASAR TRADISIONAL DAN MINIMARKET KOTA SEMARANG Ernanto, Aditya Rahman; Palimbongan, Junita Rensa; Manufandu, Anjar Richardo; Darmawati, Sri
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): (In Progress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.207

Abstract

Grass-jelly is one of the most popular plants consumed by people in various forms of beverage mix because it is easy to gets in traditional markets. Microorganism contamination can occur in grass-jelly due to several factors such as water for processing, equipments, processing facilities and from people who did the process. Contamination can cause various diseases, one of those is typhoid fever by Salmonella typhi. The purpose of this study was to detect S. typhi in grass-jelly based on molecular detection by amplification of the fliC gene using PCR. Steps of the methods were DNA extraction from grass-jelly samples, genomic DNA quality testing, amplification of fliC gene and agarose electrophoresis. Validation was done by culture methods on SSA media and biochemical testing. The fliC gene amplification results in grass-jelly samples (A1, A2, B1, B2, C1 and C3) showed the DNA fragments size about 1500 bp. Colony and biochemical characters isolates Peterongan were lead to S. typhi, whereas another isolate were another Salmonella spp. The conclusion of this study was that grass-jelly samples from Peterongan market in Semarang were positively contaminated by S. typhi and isolate from Pedurungan and minimarket were another Salmonella spp. Molecular-based food testing is fast enough and accurate for detecting types of bacterial contaminants but amplification of fliC gene cannot specific for S. typhi.
Co-Authors A.A. Ketut Agung Cahyawan W Aprilia Indra Kartika, Aprilia Indra Batara, Murni Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fandhi Adi Wardoyo Fuad, Hayatun La’lang, Marselaonety LANGKAH SEMBIRING Manufandu, Anjar Richardo Masashi Kawaichi, Masashi Nur Hidayah Palimbongan, Junita Rensa Prastiyanto, Muhammad Evy Prastiyanto, Muhammad Evy Rosnita . Rustiyarso . Silvestre, Richard David Sri Sinto Dewi Stalis Norma Ethica Sulistyaningtyas, Ayu Rahmawati Susanti, A.Meryam Wayan T. Artama Wayan T. Artama Widya Asmara Wilson, Wildiani